Hypothesis Testing Essay Example for Free

Hypothesis Testing Essay In today’s world of statistical research there is a multitude of ways to make new discoveries and formulate new ideas; one way to accomplish this is for researchers to have inquiring minds that need to have their questions answered. To do this they must develop their hypothesis. Then researchers can develop a verbal and numerical hypothesis in which they will be able to test this hypothesis. In patients with Attention Deficit Hyperactivity Disorder (ADHD), there seems to be a malfunction in the brain cells to soak up dopamine, which can lead to ADHD, it causes the patient to lose the ability to focus or retain any attention span (Allan, 2012). Team A will show the statistical outcome of diet versus traditional medications. Common treatments and side effects will be discussed in another slide, along with the importance of eating a balanced diet with â€Å"clean† foods. Results show that eating a high-protein diet with complex carbohydrates and foods with Omega -3 fatty acids seem to play a role in alleviating ADHD symptoms. What seems to do good things for our brains seems to do good things for those that suffer from ADHD. Avoiding foods that are simple carbohydrates like candy, sugars, and foods made with white flour is a good idea, as these foods seem to make matters worse. ADHD and Diet Attention deficit hyperactivity disorder, also known as ADHD, is a developmental disability that usually presents in early childhood and results in a persistent and chronic pattern of learning impairment in school, in social settings, and in daily adaptive functioning. The lasting pattern of inattentiveness and/or uncontrollability or recklessness is more often exhibited and more relentless than which is typically observed in individuals at a comparable level of physical and emotional development. In adulthood, ADHD can affect an individual’s work, behavior, social life, and relationships. ADHD tends to reflect an individual’s normal behaviors but at an exaggerated level. This causes difficulty focusing, following through on tasks, meeting deadlines, and the ability to stay motivated especially during tasks that are tedious and extraneous (Duca, 2010). Some research has shown that diet can have an effect on the symptoms of ADHD. In the past 15 or more years more information has been found linking nutrients with ADHD. Researchers are considering multiple etiologies in regards to the development of ADHD and it has become more evident that diet does play a major role in the foundation of ADHD. Studies have shown that the brain abnormalities that individuals with ADHD have been shown to have are not preprogrammed but instead are a manifestation of genetic susceptibilities to noted risk factors including diet and malnutrition. Particular individuals may be genetically heightened necessities for certain nutrients. When these individuals do not receive these nutrients in their diet they are more vulnerable to the brain abnormalities associated with ADHD (Duca, 2010). Conventionally ADHD is treated with stimulant medications such as Ritalin, Focalin, Dexedrine, and Adderall. These medications work by enhancing the transmission of nor epinephrine and dopamine in the brain but they do not come without side effects such as loss of appetite, insomnia, irritability, depressive symptoms, headaches, fatigue, and nausea. Most of the psychological and conventional medical community does not support nontraditional treatments such as nutrient supplementation, diet manipulation, and environmental and food elimination because they do not feel that there is enough research to show that these methods can permanently alter the symptoms of ADHD (Duca, 2010). From a dietary viewpoint ADHD prevention begins early in life during the first three years when brain growth is rampant. Diet plays an important role during this time to support brain health. Research has shown that the underlying contributor to the etiology of ADHD is imbalances in a child’s diet. A balanced diet free of preservatives, processed sugars, and potential food allergens is helpful in the prevention and intervention of ADHD. A diet rich in organic whole foods, fresh vegetables and fruits, abundant in mineral and phytonutrients, and rich in essential fats are important (Duca, 2010). Numerical and Verbal Hypothesis Statement Based on previously acquired research on how a healthy diet of clean non processed foods can help to lessen the symptoms of ADHD Team A has decided to test this theory to see if it is accurate. The research hypothesis that Team A has created is that: Eating a healthy â€Å"clean† diet can help to lessen the symptoms of ADHD. On the other hand the null hypothesis would claim that a healthy diet has no effect on the symptoms of ADHD and would be just as effective as if the subject ate a diet of 100% processed foods. In order to complete this test Team A will have two populations of people to research, population one will be made up of six people who are all on a diet of pre-chosen healthy foods. Population two will be made up of six people who are not following the specified healthy diet and instead eating what could be considered â€Å"junk† food or â€Å"bad† food. Team A’s hypothesis written in numerical form has to start with identifying  µ=the mean symptoms present in those eating healthy. We will also have to restate the hypothesis which would be written out as (H1)  µ1  µ2, simply meaning that the mean of population one will be less then population two. This should be able to prove that population one (the healthy eating group) will have less symptoms than population two (non healthy eating). The null hypothesis states (H0)  µ1 =  µ2 which means that population one will equal population two and there will be no difference between the groups symptoms as a result of changing their diets. Using this information we will be able to properly show a breakdown of the five steps of hypothesis testing that Team A will follow. Five Steps of Hypothesis Testing Five steps are involved in hypothesis testing. The point of the steps is to either prove or to disprove the questions involved. They are useful in evaluating the solutions of an issue or research opportunity. An example is a possible correlation between attention deficit hyperactive disorder (ADHD) and diet. The first step of process includes identification of a null hypothesis and variables involved, or more simply, stating the issue being studied. This is a comparison of two categories. Step one is this case claims that poor diet or malnutrition partnered with genetic susceptibilities potentially do not influence brain abnormalities which are associated with ADHD. The independent variable is diet and is categorical. The dependant variable is ADHD symptoms which are continuous (Davis 2007). The second step of the process involves specifying an alternative hypothesis which suggests a difference of the variables. Our null denied symptoms of ADHD influenced by diet. The alternative to this is that ADHD symptoms decrease with improved diet. Therefore, the two groups of our sample, or the two categories of diet are poor diet and good diet (Davis 2007). Step 3 of this process involves setting a significance or alpha level. In other words, determine the degree to which error is allowed. Type I is alpha and Type II is beta. The confidence level involves type I or type II errors. Whether or not diet influences ADHD symptoms is the question. When the truth is that diet indeed does influence (H0), selecting does not (Ha) would be a Type I error. If the truth is diet does not influence (Ha), then selecting that it does (H0) would be a Type II error (Davis 2007). The fourth part of the process involves calculating the probability of the hypothesis occurring. If this probability ends up being equal to our significance level (alpha/beta) then our null hypothesis that poor diet does not influence brain abnormalities associated with ADHD. If the null is rejected then the alternative is accepted but never with 100% certainty. The fifth and final step is simply describing the results of the study and stating our conclusion in relation to the original question (Davis 2007). Team A’s researcher referenced a study linking dietary needs to improve the functions and abilities for children that suffer with ADHD. Research shows that a contributing factor with ADHS is dietary imbalance in children beginning at a young age of around 3 years old. A balanced diet is said to help improve this imbalance, when the dietary needs are met using whole foods rich in mineral and phytonutrients with rich essential fatty oils (Duca, 2010). Being that the research showed underlying contributions this motivated Team A to further our investigation. Team A already had the knowledge that healthy eating led to proper body function and mechanics, but how dietary could needs improve the functions and abilities of children that suffer with ADHD is what Team A wanted to research further. Team A found that the side effects from medications lead to some disturbing results such as loss of appetite, insomnia, irritability, depressive symptoms, headaches, fatigue, and nausea. This is no way for a child to live and if symptoms can or even have the slightest ability to controlled, then proper diet with â€Å"clean† foods should be the way to proceed. References Allan, S. (2012). The Brain, Nutrition and ADHD. Retrieved from http://www.sevencounties.org/ website. Duca, R. (2010). Nutritional Considerations in the Management of Attention Deficit Hyperactivity Disorder. Nutritional Perspectives: Journal Of The Council On Nutrition, 34(4), 5-16. Davis, B.L. (2007). Decision Rule for Hypothesis Testing. Retrieved from http://home.chpc.utah.edu/ website.

Dengue Fever and the Immune System

Dengue Fever and the Immune System Dengue virus cause variable range of dengue shock syndrome with fatal dengue varies from undifferentiated. Because of the increasing incidence and geographical distribution of dengue in the past 50 years, dengue has become increasingly recognized as one of the main infectious diseases in the world. This research will study the aspects of immune system and dengue. Also offers the importance of dengue virus infection and discusses the current knowledge of dengue fever. Dengue is a disease caused by a mosquito of dengue virus. In the last century, the dengue goes up in severity of illness and the geographical distribution is now the most common infections of human Arthropod-borne viruses in the subtropical and subtropical regions of the world. Dengue is endemic in more than 100 countries around the world. It causes nearly 50 to 100 million cases per year, including 250,000 to 500,000 dengue hemorrhagic fever or dengue shock syndrome. World Health Organization reveal that the two fifth of world population is at a threat of dengue virus infection. It was stressed that globalization and climate change has a major impact on the emergence of dengue virus in new areas. Dengue is also a major economic burden on affected areas, with annual cost estimated at several billion dollars. Since there is no vaccine against dengue virus, is the primary means of preventing diseases targeting mosquito vectors and to break the cycle of transmission. This requires a large and well-organized, as well as financing of intervention programmes. Although there are programmes in countries affected, in Sri Lanka, which is currently in service in disease vector control strategies were not sufficient to combat dengue. New tools and novel approach are therefore urgently needed. The incubation period of dengue is around four days. A person about to reduce fever and to provide occasional macular or maculopapular rash. It was difficult to distinguish dengue viral diseases, person usually recovers 5 days. In the most serious cases, fever and rash are accompanied by headache, ritrorbital pain, myalgia, back pain, sore throat and abdominal pain. Becoming lethargic patients suffering from anorexia and nausea. The DHF as dengue hemorrhagic fever has similar incubation period and number same symptoms. However, the fever is more severe and the tiredness and lethargy more extreme. The patient may increase vascular permeability and abnormal balance. This can cause the individual to reduce the volume of blood, leading to low blood pressure and go in dengue shock syndrome (DSS) and die. Dengue virus infection continues to present a major and escalating global public health problem. Fight against vectors of the programs have been largely unsuccessful or local benefits in the short term only and therefore will continue to develop the vaccine is likely to be the most effective control strategy. Diseases of infection by the dengue and participation apparent protective immune response and disease has proven to be complex and difficult to understand and therefore required more research on these subjects. It is recognized that important steps to accelerate the dengue fever vaccine established an international network of centers for the clinical trial. Evaluation of vaccine experimentation is time-consuming and therefore you should not stop this process by theoretical considerations. Then only will be an initiative of the dengue vaccine in children in a position to assess the effectiveness of a variety of weapons to use in the fight against dengue. 1.0 Introduction Dengue fever is a disease caused by virus which is carried by mosquitoes. Then, these mosquitoes transmit the virus to humans. The virus that causes dengue fever has called an arbovirus, which is the short form for viruses transmitted by arthropods. Mosquitoes are arthropod type insects. Mosquitoes transmit this virus and responsible for passing it along to humans in a several areas. These countries include the Far East, Middle East, Africa, the Islands Caribbean and countries of Asia such as the India, Sri Lanka and the Thailand. In these areas, Arboviruses of dengue fever is widespread, which means that the virus naturally and repeatedly and repeatedly lives at those countries (1). To understand how the dengue fever is transmitted, several conditions must be defined. The meaning of the word host is an animal or human that can be affected with a particular disease. The meaning of the word vector is a body that can carry a particular virus causing agent such as a virus or bacteria with no actually develop the disease. The vector can then go by the virus or bacteria to a new host (2), (3). Most common diseases include the cold, many viral causes of diarrhea and flu or the influenza spread because the viruses that cause these diseases can be transmitted directly from one person to another. However, dengue fever cannot be transmitted directly from one person infected with another. The dengue virus must need an intermediate vector, a mosquito which takes the virus from one host to one more. The mosquito that takes the Arboviruses, reliable for dengue fever is the similar kind of mosquitoes that can transmit other diseases such as yellow fever. This mosquito is named as Aedes egypti. The more often victims are children less than 10 years (4). 2.0 Dengue Viral Infection When a person feeds by an infected mosquito, it injected dengue virus in the bloodstream. The virus infects close to skin cells called keratinocytes the most common type in skin cells. The dengue virus has also infected and reproduced within a specialized immune cell in the skin, a dendritic cell type called a Langerhans cell (5). Langerhans cells detect the pathogenic invaders and display molecules from the pathogens known as Antigen, on their surface. Langerhans cells then pass through to the lymph nodes and warn the immune system to prompt immune response as a pathogen inside the body. Lymph nodes are small organs that placed all the way through body, linked by vessels that form a set of connections named as lymphatic system (6). Lymph nodes are located in the body to help the fight against infection of immune cells. 3.0 The immune response When a pathogen as dengue virus invades the human body, the body to defend itself by defense of the body against pathogenic invaders is the immune system, which consists of two parts. First part is called as the innate immune system and it provides the body with urgent and regular defense to any pathogen that invading. The innate immune responses quickly identify and react against to pathogens. However it doesnà ¢Ãƒ ¢Ã¢â‚¬Å¡Ã‚ ¬Ãƒ ¢Ã¢â‚¬Å¾Ã‚ ¢t afford a person with immunity in the long term against an invasion of pathogens. Second part of the immune system called as the adaptive immune system generates cells that target more precisely and effectively towards pathogens and affected cells. The cells created by the Adaptive immune system consist of some B cells secreting antibodies and cytotoxic t cells. Immunoglobulin or Ig antibodies concealed by the cells B specially recognize and attach to foreign molecules. Cytotoxic T cells kill cells that are affected with pathogens. Adaptive immune system spends much time to react pathogens that invades the innate immune response (7), (8), but it is a person with immunity against a pathogen in long term. 4.0 Dengue Virus Tricks the immune system When the dengue virus infected mosquito bites the skin of the person, then the person also becomes a dengue infected. The dengue virus infects Langerhans cells, a type of dendritic cells in the skin. Infected cells of Langerhans produce interferons can help to limit the continued spread of the infection. Other infected cells to travel to the lymph nodes carrying viruses that infect more cells of Langerhans. Viremia is resulted by the spread of dengue virus when the high level of bloodstream affected. In order to fight against infection, the immune system construct antibodies to defuse the virus particles of dengue and the complement system is enabled for antibodies and white blood cells to get rid of virus. The immune response also consists of cytotoxic T cells also called as lymphocytes that identify and destroy infected cells. Langerhans cells once infected by dengue virus, they travel on the site of the infection of the skin to lymph nodes. The immune system responds to Infected Langerhans cells are viral antigens of dengue fever on their surface, which enables the innate immune response in alerting the two types of white blood cells called macrophages and monocytes that fight against the virus. In general, monocytes and macrophages consume and wipe out pathogens. But as an alternative of destroying the dengue virus, both of white blood cell types are pointed and infected from the virus. Dengue virus tricks the immune system to circumvent the protection and affect more cells (9). As the affected monocytes and macrophages to go through the lymphatic system, the dengue virus spreads all the way through the body. Throughout his trip, the dengue virus infects lots of cells, including lymph nodes and bone marrow macrophages of the spleen, liver and blood monocytes. Widening and the spread in the outcome of vir us Viremia is a situation in which there the bloodstream contains a high level of dengue virus. Mainly there are four different tensions of dengue virus. These strains are very similar, therefore the immune system recognizes all after seeing one. But the recognition is not protection. Each T cells of humans are automatically identified a specific model or an Antigen. In the first viral infection will be captured and processed by the above mentioned antigen presenting cells. These viruses will be open to T cells forcing them to become activated. And even the B cells will meet their floating free Antigen and make active. B cells create antibodies. They are used to tag the virus to encourage their uptake by macrophages called as opsonisation and inactivate them (10). The first infection is identified as dengue fever and lasted 6 or 7 days (11). Its symptoms closely look like as a severe flu. Most people get over it with no incident. If one person become affected from fever again things could leave in the wrong. Four types of dengue strains that all are extremely similar. If you are infected with a dissimilar strain that you met the first time, you contract dengue hemorrhagic fever. The reason for happening this is it turns out that the first infection antibodies attach to virus particles but not inactivate them. The strains are dissimilar enough to stay active in the existence of antibodies against another strain. These are called non neutralizing antibodies. These antibodies will always result in the opsonization if the Macrophages are readily transmittable virus. These speeds up the course on a point infection such as hemorrhagic symptoms are observed. It is one of those interesting times where our immune system not succeeds us. If people were vaccinated for dengue and if someone missed one out of those four vaccines has been inactivated by the improper storage, they would be very susceptible to dengue hemorrhagic fever. 5.0 Immune system Defeats the Dengue Virus Although the dengue virus has trapped immune system to infect cells and widen all the way through the body, the immune system contain further protection against the virus. Infected cells produce and release the tiny proteins named as interferons that are part of a huge collection of proteins named as cytokines. Interferons have the capability to interfere with viral replication and they activate the innate and adaptive immune defenses. They help the immune system recognizes cells infected by dengue and help protect cells not infected infection. The immune system, fight against dengue infection so the person will experience a fever (12). As the Adaptive immune response begins to fight against dengue infection, b cells produce antibodies IgM and IgG which are released into the blood and lymphatic fluid, where they specifically identify and deactivate dengue virus particles. In another immune response adaptation, killer T cells or cytotoxic T cells, recognize and kill cells infected with the dengue virus. Active innate immune response of the complement system, response that helps antibodies and white blood cells get rid of the virus. Together with the innate and adaptive immune responses defuse infection of dengue and the patient will recover from dengue (13). If there is a huge degree of virus in the bloodstream an infected person is experiencing acute dengue symptoms. As the immune response against infection by the dengue, personà ¢Ãƒ ¢Ã¢â‚¬Å¡Ã‚ ¬Ãƒ ¢Ã¢â‚¬Å¾Ã‚ ¢s B cells begin to create antibodies called IgM and IgG which are released into blood and the lymph fluid and then they identify and defuse the dengue virus and the viral such as dengue NS1 protein molecules (14). The immune response get rid of the virus and leading to recovery. 6.0 Secondary infections of Dengue After a dengue infection for the first time person is protected against infection with the three serotypes of dengue rest for several (2-3) months. Unfortunately, ità ¢Ãƒ ¢Ã¢â‚¬Å¡Ã‚ ¬Ãƒ ¢Ã¢â‚¬Å¾Ã‚ ¢s not a long-term protection, and after this short time period, person can be affected with any of the three serotypes of dengue that are outstanding. Around 1960s, Dr Scott Halstead and some of his colleagues are studying in Thailand dengue virus (15), (16). They noticed that the people who had been infected to dengue virus second time had a bigger risk of severe dengue compared with who had not been infected previously. They were asking what makes a second infection of dengue, not as well as the first. In general after an infection from a pathogen, the body keeps in mind the infection for long because the cells named as memory B cells and memory T cells remain inside the body. Since they remember the first disease, memory cells can respond quickly to provide an adaptive response, when infection occurs for the second time. Memory cells can stay behind in the body for many years or even a lifetime. These memory cells help fight a second dengue infection. More often worse than the first infection second dengue infection. Halstead proposes a phenomenon called dependent improvement of antibodies to the infection to explain these observations. There are four types of dengue (serotypes), but the cells of memory do the immunity against reinfection by serotype of dengue fever that caused the first infection. When a person is infected with a second dengue serotype, Halstead suggested that antibodies to the first infection actually help spread of dengue virus infection and increase the amount of virus in the blood, Viremia. This phenomenon can also occur in children who received antibodies against dengue of their mother while in the uterus. Curiously, rather than destroy the virus, existing antibody and the antibody newly produced by the memory can of b cells actually help the virus infected host cells more effectively. Ironically, the consequence of dependent development of antibodies is the response of the immune system in fact makes the clinical symptoms of the dengue fever situation and raises the risk o f serious diseases of dengue. In a second dengue infection cytotoxic T produced by the immune system cells provide only partial immunity against the new serotype of dengue (17). Cytotoxic T cells are not responsible for effectively remove the virus from the body and they issue some excess amounts of molecules named as cytokines. In general quantities and cytokines help out the immune response. Though in large quantities, cytokines may produce severe inflammation and tissue such as capillary leak; eventually contribute to the development of serious diseases of dengue. 7.0 Factors That Contribute to Severe Dengue Infections Prevention of dengue fever means reducing the mosquito population. Any resources of standing water such as buckets, vases, etc. where the mosquitoes can breed must be eradicated. Mosquito repellant is suggested for those areas where dengue fever is widespread. To help break the cycle of spread, sick patients those who are suffered from the dengue fever must be placed in bed net, then the mosquitoes cannot bite them so that they wonà ¢Ãƒ ¢Ã¢â‚¬Å¡Ã‚ ¬Ãƒ ¢Ã¢â‚¬Å¾Ã‚ ¢t become arboviral vectors (18). Became the prevention and the fight against dengue, dengue hemorrhagic fever and urgent distribution of geographic expansion and increase the spread of the disease over the last 20 years. Unfortunately, the tools available to prevent dengue are very less. There is a no any vaccine invented yet, and the limited option available to fight against mosquitoes. Clearly, the emphasis should be on prevention of the disease, and if the trend emerging diseases must be reversed (19). If the effective prevention of the disease and several integrated components, including active laboratory surveillance and emergency response and medical community education to guarantee successful management of the situation, mosquitoes community integrated pest and use of effective vaccines when available Disease Control active is an important part of prevention program against dengue. In addition to monitoring secular trends, the purpose of monitoring should be to give warning or predictive capability for the transfer of the epidemic, and the reason is that if you cannot predict epidemics can be avoided by starting by fighting against mosquitoes emergency. For the prediction of epidemics, health authorities and should be able to precisely control the transfer of dengue virus in the community and be able to say at any time that the virus is transmitted virus serotypes rotation and related this kind of disease with dengue infection. To do this, the system must be on site. 8.0 Causes and symptoms Dengue fever can caused when a mosquito bites a human carrying the Arboviruses and transmit the virus to a new host. When the live virus inside body, it moves to various parts where it crashed. The virus is able to then go through the bloodstream. The existence of the virus within the blood vessels causes modifications to these blood vessels. The vessels enlarge and blood leak through the vessels. The spleen and lymph nodes become swollen. A procedure called disseminated intravascular coagulation (DIC) happens, where chemicals relevant for clotting are used up and it will guided to severe bleeding which is called as hemorrhage. When the virus has been spread to the human host, happens a period of incubation. During this time it last approximately five to eight days, the virus multiplies. The signs of the disease come out at once and consist of headache, high fever, chills, eye pain, lymph nodes swollen, red eyes, back pain, red hunting in the face, extreme weakness and severe pain in the limbs and joints (20). This early duration of the disease is about 2-3 days and after this duration, fever decreases rapidly, and the patient sweats strongly. After about a day to feel relatively well, the patient temperature increases again, but not as much as the first time. Begins a rash of small red bumps on arms and legs, spread to the chest and abdomen. It hardly ever affects the face. The palms and the soles of hands and feets swollen and turn bright red. The combination of characteristic of fever, rash and headaches are named as the dengue triad. Many people get well completely from dengue fever, even if the weakness and fatigue may last numerous weeks. When a person has been suffered by dengue fever, his immune system guards produce for about a year cells that prevent reinfection from dengue virus (21). More serious diseases can occur in some people. These persons may meet with dengue fever for the first time. Anyway, in some situations, that a person may have already had dengue recovered at a time and then is re-infected with the virus. In these cases, the infection first guides the immune system to identify the attendance of the Arboviruses. When the immune cells the virus infections later, the immune system reacts. These types of disease called dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) (22), engage more strict symptoms. Fever and headache are the opening symptoms, but signs of dengue fever are not present. The patient improves a cough go after by the appearance of small purple patches named as petechiae on the skin. The petechiae are areas where the blood is leaking from the ships. Large areas bruises appear as the getting worse bleeding and abdominal pain can be strict. The patient may starts to vomit a material that is similar to coffee. It is actually a si gn of hemorrhage in the stomach (23). As the blood vessels turn into more injured, they escape more and carry on increasing in diameter called as dilation, causing a decrease in blood flow to the body tissues. This condition of reducing the blood flow is called as shock. Shock can cause damage to the organs of the body especially the heart and kidneys, because the low blood flow deprived oxygen (24). 9.0 Diagnosis Diagnosis should be detected in native areas each time that a high fever continues for 2-7 days, especially if go together with by a ability to hemorrhage. The signs of shock should suggest the development of the disease to the dengue. There are several types of Arboviruses but one of them causing dengue fever that can be isolated from the blood serum (25), (26). The serum is the fluid that the blood cells are floating. Serum can be verified because the phase in which the virus moves into the bloodstream is longer in DF than in other arbovirus diseases. Numerous tests are used to find reactions between the serum of the patient and antibodies produced in the laboratory. Antibodies are particular cells that identify the markers or antigens present on the attack of the body. During these tests, the antibodies are mixed with a sample of the patient serum. Health care workers and then looks for reactions that would occur only if the virus antigens are present in the serum (27). 10.0 New diagnostic technology In recent years, he has developed several new methods of diagnosis and has proved very useful in the diagnosis of dengue. After reviewing this issue in depth over the past. Here follows a brief description of the various methods below. 10.1 PCR Reverse transcriptase PCR (RT-PCR) was build up for numeral RNA viruses in recent years and possible to modernize laboratory diagnosis of dengue (28). RT-PCR provides a rapid diagnosis specific serum. This method is fast, simple, sensitive and reproducible if handled properly it can be used to discover viral RNA in human clinical specimens and autopsy tissues, or mosquitoes. There were number of methods developed involving primers genome in different places and different methods to detect RT-PCR products in recent years (29). 10.2 Immunohistochemistry A major problem in the laboratory diagnosis of dengue confirmation of fatal cases. Only one sample is obtained serum and serological tests of limited value. In addition, most patients die at the same time or shortly after defervescence as the virus isolation is difficult (30). With novel techniques of building, it is now possible to detect antigens of dengue viruses in a variety of tissues. 11.0 Treatment No treatments are available to reduce the course of dengue hemorrhagic fever or dengue shock syndrome. Drugs to reduce fever and decrease muscle pain and headache pain (31). Fluids are given through a needle into a vein to prevent dewatering. Blood transfusion may be necessary if serious bleeding happens. Oxygen should be given to shocked patients (29). 12.0 Conclusion The immune system is the bodys defense against viruses dengue Home. When a person is infected with the dengue, response to innate and adaptive immune system to join forces to fight against the virus. B cells create antibodies which specifically identify and defuse foreign particles and viral cytotoxic T cells to recognize and destroy cells that are infected by the dengue virus. For people who can develop later with another type of dengue virus experience called antibody based on the promotion. This situation occurs when the immune response leads one clinical symptoms of dengue worse, which increases the risk of severe dengue.

My Last Duchess by Robert Browning Essay -- Last Duchess Robert Browni

My Last Duchess by Robert Browning In his poem â€Å"My Last Duchess†, Robert Browning gives his readers a complex picture of his two main characters. The Duke, who narrates the poem, is the most immediately present but Browning sets him up to ultimately lose the reader’s trust. The Duchess becomes the sympathetic character, a victim of foul play. It is through the various representations of the Duchess within the poem that we come to know both characters. The representations of the Duchess, which focus on her ever-present smile and easily satisfied nature, come in sharp contrast with the desperate, sputtering language of the Duke as he tries to tell their story on his own terms. This contrast is a manifestation of the Duke's frustration with his inability to control the Duchess and her nonchalant but near-total control over him. The Duchess is first introduced as a painting hanging in the Duke’s gallery. The very form in which we meet her gives us an indication of both her passivity and her ability to persist, unchanged, in one mode of behavior. A painting has very little living communicative power, relying on the expressiveness of its subject at the time of painting. It is notable that no mention is made of any background or accompanying objects in the painting—often in portraiture these elements are relied upon to convey key ideas about the subject. It seems that the Duchess relied solely upon herself and the painter to tell her own story. Even if other objects are in the painting, they are unremarkable enough that neither Duke nor poet feels compelled to mention them. From a literary standpoint, this means that the poet felt that we needed no other initial information about the Duchess. Even at the level of chara... ...haunts him, and by placing it both first and last he drives it home very strongly. He can’t help but repeat that phrase when confronted with the Duchess who is both still smiling and â€Å"as if alive†Ã¢â‚¬â€he is driven mad by the idea that he couldn’t even succeed in killing her. His actions, too, are driven by the Duchess. Since she is still smiling and life-like, despite his best efforts to the contrary, he is driven to the irrational extreme of covering the painting and ensuring that â€Å"none puts by the curtain†¦but [himself]† (9-10) His extraordinary desire to control the Duchess leave him vulnerable to her imperviousness. By remaining unaffected by the Duke’s strenuous efforts to alter her behavior, the Duchess forces the Duke to take more and more drastic measures—like killing her and hiding her painting—and eats away at his ability to even keep control of himself.

Lord of the Flies Chaos vs Order Essay

The conflict between two competing impulses that exist within all human beings: the instinct to live by rules, act peacefully, follow moral commands, and value the good of the group against the instinct to gratify one’s immediate desires, act violently to obtain supremacy over others, and enforce one’s will. This conflict might be expressed in a number of ways: civilization vs. savagery, order vs. chaos, reason vs. impulse, law vs. anarchy, or the broader heading of good vs. evil. Throughout the novel, Golding associates the instinct of civilization with good and the instinct of savagery with evil. Topic sentence isputed leadership and infighting between the two dominate powers (Jack and Ralph) cause a disputed leading to the separation of the tribe into two separate tribes each determined to destroy and ruin the other showing how government (power) can deteriorate to nothing if the following â€Å"citizens† don’t go along with the ruling power they started out with a good governing system based mostly off of a dictator ship but allowing some voting as in democracy this lead to the group working together to build houses, gather food, create weapons and finally set up fires for both cooking and signaling a passing ship. This system worked for the majority of the book allowing the children to solve their problems as a group effort with all contributing to what they could. The evidence showing this is as follows, when they first landed on the island the idea of government was first brought up by the conch shell which symbolized power and eventually lead to the election of a leader (Ralph), leading to the children coming together and working towards survival and hopefully their salvation this as we know failed as the tribe split in two and became democratic and dictatorship ased with Ralph as the democratic leader and Jack as the Dictatorships leader. Ralph and Piggy discover the conch shell on the beach at the start of the novel and use it to summon the boys together after the crash separates them. Used in this capacity, the conch shell becomes a powerful symbol of civilization and order in the novel. The shell effectively governs the boys’ meetings, for the boy who holds the shell holds the right to speak. In this regard, the shell is more than a symbol—it is an actual vessel of political legitimacy and democratic power. As the island civilization erodes and the boys descend into savagery, the conch shell loses its power and influence among them. Ralph clutches the shell desperately when he talks about his role in murdering Simon. Later, the other boys ignore Ralph and throw stones at him when he attempts to blow the conch in Jack’s camp. The boulder that Roger rolls onto Piggy also crushes the conch shell, signifying the demise of the civilized instinct among almost all the boys on the island. Now on to the dictatorship Jack set up with his symbol The sow’s head is a symbol of savagery and destruction. The boy who introduced the idea of the sow’s head on a stick was Jack, and Jack too is a symbol in the book. Jack stands for savagery. You know right from the start of the novel the Jack is not like the other boys, and that he’s a savage because of what he tells Ralph that he wants him and his choir to be. When asked what he would like to do on the island he replies â€Å"Hunters† showing he only cares for killing and wants to be the only provider on the island this then gives Jack the theoretical power he wanted from the start (the leadership of the tribe) The power the sow’s head holds over the boys is more of fear than a power. The sow’s head is a constant reminder that they are living like savages. I think it also reminds the boys that if they can kill a pig then they would probably kill one of the others. This once again shows how Jack is willing to kill for his power and take what he wants from Ralph (later shown when he splits the tribe and attempts to become supreme ruler) To Simon the sow’s head holds a completely different power over him. To Simon the sow’s head represents craziness. Simon is different than the other boys, so that might by why the sow’s head is different for him. When the other boys are fighting, Simon goes to his private glade in the forest. The thing that gave me the idea that the sow’s head represents something different to Simon is because Simon has a conversation with it. Also because the conversation sounds like something that would’ve came out of Simon’s head, because Simon never really believed in a beast on the island and after learning the truth sets off to tell the others ultimately leading to his own demise as the boys mistake him for â€Å"the beast† and kill him during a ritual.

Ethiopian Womans Rights Essays

Ethiopian Womans Rights Essays Ethiopian Womans Rights Essay Ethiopian Womans Rights Essay In the societal structure of Ethiopia that the women have grown so accustomed to, the culture is now key in determining womens roles. Ethiopian women are actively going through hardship in the experience of their life. They have lagged behind men and have been restricted from being free from social, economic and political rules and regulations. Even the civil code of the country confirmed womens inferior position. In the faithful attempt of trying to even the rights of women, the Women in Development group sought to assist the women of Ethiopia by implementing the National Policy on Women. The terms of this policy aim to create appropriate structures within government offices and institutions to establish equitable and gender-sensistive public policies. Among other attempts, Meaza Ashenafi was one of the privileged women of Ethiopia in the Ethiopian Women Lawyer Association, that stood up for what she believed in and put forth practices for over 300 women to undergo paralegal training for basic Ethiopian law. Her idea is to empower women and allow them to use the information for themselves to be able to speak and defend and fight for equality. She envisioned more educated women, which was also the goal of the Revolutionary Ethiopia Womens association, in advocacy of actively educating women. Ethiopia also takes on a physical heavy toll on women and even the children residing in the rural areas which is over 85%, where it is labor intensive. As far as their future can go, there are priorities that can be subdued in the country to improve womens lifestyle in Ethiopia. Realistic steps such as improving the level of income by facilitating opportunities and woman-friendly conditions could benefit, women in some economic sectors sum up a larger workforce than men but since their participation is not valued, they have not received their fair share of the nations wealth. Its essential and practical to improve the health and nutrition of mothers and as well as improved education for both them and their children. These are only small practical steps though that are put forward from Pathfinder International, United Nations Population Fund amongst other small organizations Even with all the attempts to appease to women and their lifestyle and small organizations trying their best to help. Ethiopia truly has potential to change its perspective on women, yet due to the deep-rooted traditional values and beliefs, it could take much longer to bring that of inequality to justice. A real change would require more than providing room for short-term improvements, it would take a change in government attitudes and turning culture around. In a less developed country, its important to forward recommendations to allow not only for women to live as equals amongst men but to create a progressive society in which women are not vulnerable to domestic violence, sexual exploitation and extreme labor as well as having access to health and maternal care and education to be free from that of culturally-enforced subjugation.

11 Unethical Interview Questions You Dont Need to Answer

11 Unethical Interview Questions You Dont Need to Answer Yes, it’s an interview and you’re trying your best to be a people-pleaser. But that doesn’t mean letting someone ask you information that’s off-limits. Sometimes â€Å"innocent† questions about your hobbies and your kids can just  feel like small talk, but are secretly a trap to get you to divulge information that could affect your chances. Asking certain things outright can be considered discrimination and is explicitly not allowed by government regulation.Be vigilant for the following 11  unethical interview  questions and have a swerve answer prepared.  The best rule of thumb? If there’s a question that doesn’t seem relevant to your duties or performance or how well you’d fit into the company or role, dodge it.1. â€Å"What does your husband/wife do?†Some states actively prohibit discrimination on the grounds of marital status. These types of questions are usually asked in order to discriminate against women, w ho might become pregnant or have child care concerns that single candidates wouldn’t. Avoid answering anything that seems to assess whether you have a spouse at all.2. â€Å"Are you pregnant?†First of all, it’s rude to ask. Second, refer to the above. Any question of your kids, your plan to eventually have kids, or your childcare routines are to be avoided for the same reasons.3. â€Å"Do you have a disability that might interfere with your job performance?†(Or anything about a disability or a pattern of illness, i.e. sick days, workers’ comp claims, mental health problems, etc.) These might seem like valid questions, but it is illegal to ask any questions which would reveal the existence of a disability before the job offer is made.4. â€Å"Have you ever been arrested?†Again, some states explicitly prohibit this. And in some cases, questions like these are used to discriminate against minority candidates- which is even worse than just discr iminating against felons, and actually illegal.5. â€Å"What year did you graduate?†This is a sneaky way of figuring out your age. And age discrimination is not a good thing. It’s been federally prohibited since 1967 (at least as applied to people over the age of 40). Bottom line: you don’t ever have to disclose your age.6. â€Å"Are you in a union?†You have a right to join a union and it is not okay to try and prohibit you from doing so. You should not be questioned about your union membership or intention to acquire one, so don’t answer any questions that could give that information out.7. â€Å"Would you take a genetic test?†It is totally illegal to ask you for genetic information, as of 2008. It is also illegal to ask about your family members. You are not in any way required to take any test or give your potential employer any information about your genes.8. â€Å"Where is your name from?†These questions are aimed at figuring out your ethnic background. Other questions like this are â€Å"What other languages do you speak?† or â€Å"What was your first language?† Racial discrimination is to be frowned upon. Don’t be a party to it.9.â€Å"Where do you worship?†Or: â€Å"What days do you worship?† This might be evidence of your employer trying to figure out what your faith is. They might be legitimately curious on a personal level, but you are not required to- nor should you- say. It could be a potential means of religious discrimination- and illegal according to the Civil Rights Act.10. â€Å"Will you take a polygraph?†Most employers are federally not allowed to ask you to take a lie detector test as a condition of employment- or to be fired, not hired, or disciplined for refusing. You can thank the Employee Polygraph Protection Act of 1988 for this.11. â€Å"What do you currently make?†There’s a new equal pay law in Massachusetts that makes asking this illegal. But you’re not required to answer it anywhere else either! Try pivoting instead with a line like, â€Å"I’d prefer to discuss money a little later; right now I’m just interested in whether this is a good fit for both of us. I’m sure we’re on the same page.†

Free Essays on Absolute Monarchy

An absolute monarchy is a form of government where the ruler has the power to rule their country and citizens freely with out any laws or legally organized opposition telling the monarchy what to do, although some religious authority may be able to influence the monarch. Basically an absolute monarch has total power over its people and land which includes the aristocracy. The political theory which forms the basis of absolute monarchy was that the monarch held their position by the grace of God and was therefore not answerable to anyone but God. Absolute monarchy developed in the late Middle Ages from feudalism during which monarchs were still first among the nobility. The power of the monarch is limited by the need to have some measure of support by the aristocracy. The aristocracy would be subordinate to the monarchy, provide political and military support for the monarchy, yet may also, from time to time, challenge its authority. Much of the attraction of absolute monarchy in the Middle Ages was that it promised an end to civil wars, could put an end to corruption by the aristocracy, and restore attention to the Church's moral codes. The political thought of the Enlightenment was influenced by philosophers such as Jean Bodin, Thomas Hobbes, and Jacques-Benigne Bossuet. Jean Bodin was a French Renaissance philosopher and one of the first thinkers to defend absolutism. He wrote Six livres de la RÃ ¨publique in 1576 (Six Books of the Republic). He argued how Kings should be given the right to rule over everyone of his subjects and the political institutions they had by unrestrained laws. However, he also limited the power by saying rulers should be held by social customs and natural law. Thomas Hobbes believed in the theory of absulte monarchy. Thomas had lived through the English civil wars and the execution of Charles I in 1649 which had strengthened his resolve that absolutism was the most logical and desirable... Free Essays on Absolute Monarchy Free Essays on Absolute Monarchy An absolute monarchy is a form of government where the ruler has the power to rule their country and citizens freely with out any laws or legally organized opposition telling the monarchy what to do, although some religious authority may be able to influence the monarch. Basically an absolute monarch has total power over its people and land which includes the aristocracy. The political theory which forms the basis of absolute monarchy was that the monarch held their position by the grace of God and was therefore not answerable to anyone but God. Absolute monarchy developed in the late Middle Ages from feudalism during which monarchs were still first among the nobility. The power of the monarch is limited by the need to have some measure of support by the aristocracy. The aristocracy would be subordinate to the monarchy, provide political and military support for the monarchy, yet may also, from time to time, challenge its authority. Much of the attraction of absolute monarchy in the Middle Ages was that it promised an end to civil wars, could put an end to corruption by the aristocracy, and restore attention to the Church's moral codes. The political thought of the Enlightenment was influenced by philosophers such as Jean Bodin, Thomas Hobbes, and Jacques-Benigne Bossuet. Jean Bodin was a French Renaissance philosopher and one of the first thinkers to defend absolutism. He wrote Six livres de la RÃ ¨publique in 1576 (Six Books of the Republic). He argued how Kings should be given the right to rule over everyone of his subjects and the political institutions they had by unrestrained laws. However, he also limited the power by saying rulers should be held by social customs and natural law. Thomas Hobbes believed in the theory of absulte monarchy. Thomas had lived through the English civil wars and the execution of Charles I in 1649 which had strengthened his resolve that absolutism was the most logical and desirable...